Before searching, please read our
instructions. Currently,
Class0.0 and Class1.0 gRNA spacer sequences, which are expected to be highly specific for the CRISPR-Cas9 mediated genome editing, will be displayed for selected region or gene. All classes of gRNA spacers are available in the
JBrowser. Search requests for regions longer than 30kb will not be accepted to maintain server performance.
Searches of bigger genes or longer regions may take more time to download the results. The search function require your web browsers to support JavaScript (Latest version of Firefox is recommended).
Two RGE vectors developed by Yang's Lab are available via Addgene (
http://www.addgene.org/Yinong_Yang/).
Our polycistronic-tRNA-gRNA / Cas9 mediated RGE is available now.