CONTENTS
- PREREQUISITES
- HOW WebFCMP WORKS
- FILE FORMATS & CONFIG
PREREQUISITES
Standalone FPC program
--
http://www.genome.arizona.edu/software/fpc/
HOW WebFcmp WORKS
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The setup.pl script writes the necessary files in
<HTML_DIR_PATH>/WebAGCoL/<MAP_NAME>/WebFcmp
and <CGI-BIN_DIR_PATH>/WebAGCoL/WebFcmp
An index.html that displays the initial form page for WebFcmp for clone names input.
A fcmp.cgi cgi-scripts that runs the FPC in batch mode and displays the comparison results
to the browser.
WebFcmp uses 'clonenames.txt' file which contains clone names and indexes into the FPC .cor file.
The ScriptDir/clonenames.pl reads the FPC file and creates the file 'clonenames.txt' in
<HTML_DIR_PATH>/WebAGCoL/<MAP_NAME>/Data directory.
When a set of clones are submitted, the CGI script fcmp.cgi executes FPC in batch mode,
and outputs the results into a temporary 'tmp' directory which is then displayed in browser.
FILE FORMATS & CONFIG
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There are often multiple libraries in a FPC file, so there is a drop-down
menu on the WebFcmp that allows the user to select the library to assist in getting the
clone name. For example, our rice FPC is made
of OSJNBa and OSJNBb. In FPC, we leave off the OSJNB and just prefix
each clone with a or b. The LIBRARY_NAME variable in config file lets you specify the prefixes,
e.g. we use prefixes a and b for rice. These values will be shown in
the Library drop-down menu.
Note: If the Library naming schemes are different then you need to modify the
clonenames.pl in ScriptDir directory and fcmp_plate.cgi in /WebAGCoL/WebFcmp
directory to parse the plate names.
There is no input file for this tool.