AGCoL
Arizona Genomics
Computational Laboratory

Tucson, AZ

WebFcmp Site Manual
WebAGCoL | WebFcmp Users Manual | FPC

WebFcmp is a tool to compare a FPC clone against all others clones.

CONTENTS

  1. PREREQUISITES
  2. HOW WebFCMP WORKS
  3. FILE FORMATS & CONFIG

PREREQUISITES

  • Standalone FPC program -- http://www.genome.arizona.edu/software/fpc/
  • HOW WebFcmp WORKS   Back to top

    The setup.pl script writes the necessary files in <HTML_DIR_PATH>/WebAGCoL/<MAP_NAME>/WebFcmp and <CGI-BIN_DIR_PATH>/WebAGCoL/WebFcmp

  • An index.html that displays the initial form page for WebFcmp for clone names input.
  • A fcmp.cgi cgi-scripts that runs the FPC in batch mode and displays the comparison results to the browser.
  • WebFcmp uses 'clonenames.txt' file which contains clone names and indexes into the FPC .cor file. The ScriptDir/clonenames.pl reads the FPC file and creates the file 'clonenames.txt' in <HTML_DIR_PATH>/WebAGCoL/<MAP_NAME>/Data directory. When a set of clones are submitted, the CGI script fcmp.cgi executes FPC in batch mode, and outputs the results into a temporary 'tmp' directory which is then displayed in browser.

    FILE FORMATS & CONFIG   Back to top

    There are often multiple libraries in a FPC file, so there is a drop-down menu on the WebFcmp that allows the user to select the library to assist in getting the clone name. For example, our rice FPC is made of OSJNBa and OSJNBb. In FPC, we leave off the OSJNB and just prefix each clone with a or b. The LIBRARY_NAME variable in config file lets you specify the prefixes, e.g. we use prefixes a and b for rice. These values will be shown in the Library drop-down menu.
    Note: If the Library naming schemes are different then you need to modify the clonenames.pl in ScriptDir directory and fcmp_plate.cgi in /WebAGCoL/WebFcmp directory to parse the plate names.

    There is no input file for this tool.

    Email comments to vishal@genome.arizona.edu