CRISPR-PLANT

Welcome to CRISPR-PLANT!
CRISPR-PLANT helps researchers using the CRISPR-Cas9 system for plant genome editing.

Working with a different genome or Cas variant? You can clone the pipeline used for analysis from our GitHub repository and customize it based on your needs!

What is CRISPR-Cas9?

CRISPR-Cas9 is a type II CRISPR-Cas system. The CRISPR-Cas9 system from Streptococcus pyogenesis a simple and versatile tool for RNA guided genome editing (RGE) in different organisms. During Cas9 mediated RGE, a single or duplex short RNA molecule (guide RNA or gRNA) directs Cas9 to target the desired DNA site for genome modification or transcriptional control. The gRNA-Cas9 complex recognizes the targeted DNA by gRNA-DNA pairing between 5’-end leading sequence of gRNA (referred as gRNA spacer) and one DNA strand (complementary stand of protospacer). Cas9 also requires the presence of protospacer-adjacent motif (PAM) in the target site following the gRNA-DNA pairing region. The CRISPR-Cas9 system can be reprogrammed to target any DNA site with a PAM by exchanging the approximate 20 nt long gRNA spacer sequence. But the gRNA-Cas9 complex also recognizes PAM sites that match the gRNA spacer imperfectly, resulting off-target risk in genome editing. With this website, we provide a resource of highly specific gRNA spacer sequences for seven different plant species.

What is CRISPR-PLANT

CRISPR-PLANT is a platform to help researchers to design and construct specific gRNAs for CRISPR-Cas9 mediated genome editing in plants. In this database, gRNA spacer sequences with specificity information are available for seven model plant species or crop species.

Click here to start using CRISPR-PLANT v2.

Click here if you are new to CRISPR-Cas9.

09/27/2018 Added information to CRISPR-PLANT GitHub repo

11/01/2017 Update to CRISPR-PLANT v2 by BM and JWZ

10/21/2013 CRISPR-PLANT 0.1 was online.

10/15/2013 Demo version was set up.